What are the methods of testing for pathogens?
Chlamydia antigen tests are performed by:
1. Pathogen-specific antigen detection
It is to detect the antigen to be tested in the patient's serum and other body fluids with known antibodies, and detect the unknown pathogen antigen in the specimen with the help of immunofluorescence technology, enzyme-linked immunoassay technology, chemiluminescence technology, latex agglutination test, convective immunoelectrophoresis and other technologies, and its diagnostic value often varies depending on the specimen.
Specific pathogen antigens were detected in sterile body fluids, blood and other specimens, which has diagnostic significance. If a variety of normally resident organisms are present in the specimen, the diagnosis may be uncertain due to the presence of cross-antigens.
2. Nucleic acid detection of pathogens
The nucleic acid detection technologies commonly used in clinical practice mainly include polymerase chain reaction, nucleic acid probe hybridization technology and real-time fluorescence quantitative PCR technology.
3. Pathogen isolation and culture examination
It is a key step in the diagnosis of microbiology examination, according to the clinical symptoms, signs and microscopic examination to make a preliminary judgment of the etiology, select the most appropriate culture method, mainly to select the appropriate medium, determine the incubation conditions, and then according to the nature of the colony and the morphology of the bacteria, staining, bacterial biochemical reaction results and the identification of the separation, is a commonly used test method in clinical practice.
Sputum, urethral secretions, vaginal or cervical secretions can be collected for chlamydia culture or one of the PCR technical examination items, which can determine whether there is chlamydia, mycoplasma pneumonia, non-gonococcal infectious urethritis,vaginitis、CervicitisWait. In recent years, PCR technology has developed the detection of constant temperature amplification technology, which is fast and efficient.