How can mycoplasma be detected? These methods can do it!

Nowadays, many hospitals have methods to check for mycoplasma, and there are many ways to check, such as blood routine can detect the carriage of mycoplasma, in addition, serological examinations, X-ray examinations and smear microscopy can also be done.

1. Blood routine

Peripheral white blood cell count is usually normal, and eosinophilia is present.

2. X-ray examination

Chlamydia pneumonia chest x-ray is nonspecific, mostly unilateral lower lobe infiltrates, manifested as segmental pneumonia, and in severe cases, extensive bilateral pneumonia. Chest x-ray of Chlamydia trachomatis pneumonia shows extensive bilateral interstitial and alveolar infiltrates, hyperinflation is common, and lobar consolidation is occasionally seen.

3. Direct smear microscopic examination

Samples of pharyngeal secretions, sputum, respiratory tract membranes or other parts were taken for smear staining, and GZmesa staining was performed, and the original body was stained red, and the original body was stained dark blue. Chlamydia trachomatis inclusions are known for their glycogen content. 801 dyed brown.

4. Rapid antigen test

Monoclonal antibody direct immunofluorescence method is mostly used to detect chlamydia in specimens, and anti-chlamydia antibody, enzyme labeled antibody 18G and substrate can also be added by 2I-IsA method for colorimetric quantitative detection. Both methods are simple and sensitive.

5. Chlamydia isolation

Chlamydia pneumoniae culture is best done with Hela cells or Hep-2 cells, and tracheal or nasopharyngeal aspirates are generally taken as clinical specimens and inoculated in time. Chlamydia is mostly identified by specific monoclonal fluorescent antibody (MFA) after HeLa cell or Hep-2 cell culture, which is highly sensitive and specific, and can obtain positive results within 48 hours if the specimen can be collected early.

6. Serological examination

The use of complement fixation assay indicates that serum antibody titers in the convalescent phase are 4 or more times higher than those in the acute phase, which is diagnostic but not early diagnosis. Micro-immunofluorescence (MrF) is indicated for Chlamydia trachomatis.

7. pCR technology

The detection of Chlamydia pneumoniae-specific DNA by ordinary pCR technology has the advantages of rapidity, simplicity and specificity, and its sensitivity is higher than that of cell isolation technology, and the detection of cheated pCR (npCR) can significantly improve its sensitivity.